Triple Marker Test

Serve Only in Pune!
5.00 out of 5
(2 customer reviews)

2,499.00 Only
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Offer from Sunday 31st May to Monday 31st March

Triple Marker Test (Triple Marker Screening Test)

2nd Trimester triple marker screening test. Latest USG report is required to undergo triple marker test.

Triple marker test is performed in pregnant women at the end of the first trimester and at the beginning of the second trimester. This test is used to detect any genetic disorders in the fetus.

Note: Read test details, FAQs and test conditions before proceeding to booking.


Not More Than 4 days Old
(For Home Visit Service, Minimum Total Test(s) Charges Should be ₹999)
(Confirm Slot Availability Before Choosing Time)

1) Home Collection Facility Available Only For Minimum Total Test(s) Charges Of ₹999.00 and above.
2) We Serve Only in Pune and Pimpri Chinchwad City Limits.
3) Additional Charges For Serving Outside Pune and Pimpri Chinchwad City Limits. (With in Pune)
4) Home Collection Facility Service Timing Should Be Selected Between 7.30am till 11.00am.
5) Direct Walk-in Service Timing Should Be Selected From 9.00am till 6pm (Monday-Saturday). Sunday from 9.00am till 1.30pm.
6) Confirm The Time Slot Availability Before Booking. There is No Auto Slot Blocking In Online Test Booking. You Should Reach to Use Over WhatsApp Or Call For Slot Availability.
7) We don't Provide Home Collection Service Only For Urine or Stool Test. These Tests Can Be Combined With Other Blood Tests.
8) Booking Date And Time Should Be 24hrs-72hrs in Advance

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Triple Marker Test

Triple marker test do in Second Trimester.

When is the triple marker test performed?

Triple marker test is performed in pregnant women at the end of the first trimester and at the beginning of the second trimester. This test is used to detect any genetic disorders in the fetus. It measures the levels of estriol, human chorionic gonadotropin (HCG), alpha-fetoprotein (AFP).

These three are important substances in the placenta. This is mostly recommended in pregnant women who had a family history of birth defects or birth disorders, women above 35 years of age, considering the weight of women, diabetes, ethnicity, etc.

What is the importance of the triple marker test?

Estriol: It is an estrogen that comes from the placenta and the fetus. If the estradiol levels are low, it may show Down’s syndrome. Down’s syndrome is the delay of growth, development, and intelligence because of chromosome 21 disorder. This will be more prominent if the alpha-fetoprotein is low and human chorionic gonadotropin levels are high.

Human chorionic gonadotropin (HCG): This is a hormone produced by the placenta. If human chorionic gonadotropin levels are decreased, it may show problems with pregnancy, like miscarriage or ectopic pregnancy (where the fetus grows outside the uterus mostly in the fallopian tube). If the human chorionic gonadotropin levels are increased, it may show a molar pregnancy or pregnancy with two or more children called multiple pregnancies.

Alpha-fetoprotein (AFP): This is a protein produced by the fetus. If this alpha-fetoprotein level is raised, it may be because of potential defects like neural tube defects or failure of the fetus’s abdomen to close.

Hence, this test helps in detecting if the baby is suffering from any genetic disorders like Down’s syndrome or Edward syndrome. Edward’s syndrome is a condition that leads to severe developmental delays because of a chromosome 18 abnormality. This is a very beneficial method to detect any genetic defects in the baby before their birth.

This test provides a probable ratio of the unborn baby having a chromosomal defect. If the ratio is high, then further tests like amniocentesis should be carried out.

  1. Unconjugated Estradiol (E3)
  2. BHCG
  3. Alpha-fetoprotein

Triple marker test conditions


  • Do In 2nd Trimester Of Pregnancy
  • Latest USG Report Required
  • Overnight fasting is not required
  • Doctor’s prescription may be needed
  • The test result may vary depending on age, health history, etc.
  • Ideal for pregnant female above 35yrs
  • Before interpreting the results, one should keep in mind that this is not a diagnostic test but a screening test.

Also known as Triple Marker Second Trimester Blood, Triple test, Triple Marker Test 2nd Trimester, Triple Marker – Second Trimester,

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2 reviews for Triple Marker Test

  1. Vivek Nair

    Very transperant advice before going for test.

  2. 5 out of 5


    This lab not only for business interest but also for good patient service. Good luck

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