Serum protein electrophoresis – SPEP Test, Normal Ranges, Symptoms And Conditions

great wealth is health at healthcare nt sickcare
Share on facebook
Share on twitter
Share on linkedin

Serum protein electrophoresis (SPEP) is a laboratory technique that’s used to determine the levels of some types of proteins in a blood sample. There are a number of reasons why a doctor may order this test. Serum protein electrophoresis is used to help diagnose and monitor a variety of different diseases or disorders that have abnormal proteins or protein levels. Electrophoresis is not usually used by itself to diagnose a disease. Instead, it’s used along with other laboratory tests to provide more information to help with diagnosis.


Serum is the liquid part of your blood. Blood appears to be a single substance to the naked eye. However, blood has several components. Both types of blood cells (red and white) and platelets are solids. When these are removed, a liquid is left behind. This is serum.


Proteins are substances made of small chemicals called amino acids. They have a number of roles:

  • They provide structure to the body.
  • They help transport nutrients.
  • They help the body fight off disease.

Too much or too little protein can cause problems. The five groups of proteins usually considered during the Serum protein electrophoresis test are:

Albumin: This protein transports substances and plays a role in tissue growth and repair.

Alpha-1 globulins: The major alpha-1 globulin is called alpha-1-antitrypsin, which is produced by the lungs and liver and increases with inflammatory diseases.

Alpha-2 globulins: This class of protein has many functions in the body and is involved in inflammation.

Beta globulins: These proteins move substances, support immunity, and increase in number in multiple myeloma and conditions like high cholesterol and atherosclerosis.

Gamma globulins: These support the immune system and are increased in multiple myeloma, as well as some autoimmune conditions like rheumatoid arthritis and systemic lupus erythematosus.


Electrophoresis is a lab technique used to separate groups of proteins in blood serum. This allows them to be measured and analyzed individually. It involves exposing serum placed in a special type of gel to an electric current. This causes the different types of proteins to move and group together. The proteins create separate bands on the gel, which are then analyzed by the laboratory.

Symptoms and Conditions for Serum protein electrophoresis

Your doctor advise Serum protein electrophoresis if you are experiencing symptoms of a condition affecting the proteins in your blood serum. These symptoms could include the following:

  • unexplained weight loss
  • bone pain or frequent fractures
  • fatigue
  • weakness
  • nausea
  • constipation
  • excessive thirst
  • back pain
  • Some of the conditions that could be causing these symptoms are:
  • cancer
  • thyroid problems
  • diabetes
  • anemia
  • liver diseases
  • malnutrition
  • certain autoimmune diseases
  • multiple sclerosis

Normal Range of Serum protein electrophoresis values

The following table shows what most labs would consider normal results for Serum protein electrophoresis testing. These values may vary slightly from facility to facility.

Type of ProteinNormal Value – Amount of Protein
albumin3.8–5.0 (grams/deciliter)
alpha-1 globulin0.1–0.3 (grams/deciliter)
alpha-2 globulin0.6–1.0 (grams/deciliter)
beta globulin0.7–1.4 (grams/deciliter)
gamma globulin0.7–1.6 (grams/deciliter)

Learn About: 24-hour Urine Test

The various proteins in the body perform different functions. This means that high or low levels of the five protein types examined during the test can point to different illnesses. Keep in mind that these are just clues. Further investigation will usually be needed to make a definitive diagnosis.

healthcare nt sickcare

Get Us IN +919766060629

All material copyright healthcare nt sickcare. 2017 – 2019. Terms and conditions & Privacy Policy of use. The contents herein are for informational purposes only. Always seek the advice of your physician or other qualified health providers with any questions you may have regarding a medical condition. Source: This article inspired from various online articles and own offline experiences. The content meant for public awareness and regular post to the clientele of healthcare nt sickcare.

Know How We Give-Back

Create My Account

Shop With Us

Offer Of The Month

protein, protein synthesis, high protein diet, protein in urine, gel electrophoresis, agarose gel electrophoresis, alpha fetoprotein, serum globulin, globular protein, globulin high, polyacrylamide gel electrophoresis, 2d gel electrophoresis, types of electrophoresis, serum protein, serum test, protein in blood, protein protein interaction, serum globulin high, what is electrophoresis, paper electrophoresis, gamma globulin, protein electrophoresis, page electrophoresis, serum electrophoresis, agarose gel electrophoresis principle, serum total protein, dna fragmentation, electrophoresis test, serum globulin low, 2d electrophoresis, spep test, electrophoretic techniques, spep, dna electrophoresis, immunofixation electrophoresis, gel electrophoresis procedure, agarose gel electrophoresis procedure, electrophoresis uses, serum protein normal range, serum globulin normal range, electrophoresis unit, pulse field electrophoresis, application of agarose gel electrophoresis, dna gel electrophoresis, gel electrophoresis process, uses of gel electrophoresis, instrumentation of electrophoresis, electrophoresis meaning, what is serum globulin, globulin normal value, globulin high means, beta globulin, native gel electrophoresis, normal serum protein level, how to increase globulin levels, types of gel electrophoresis, gel electrophoresis steps, immunofixation serum, alpha globulin, serum protein level, serum protein

Subscribe to our Latest Blogs

Get Instant Notification Of Our Blogs On Preventive Healthcare, Wellness, Pathology Laboratory, Diseases And Disorders. 

Share this article and spread the care

Share on facebook
Share on pinterest
Share on twitter
Share on linkedin
Share on tumblr
Share on whatsapp

Leave a Reply