Pathology Laboratory

FSH LH Prolactin Blood Test

Last updated on January 21st, 2023 at 11:57 am

Do you have questions about fertility or infertility? In this article, we’ll discuss the FSH LH Prolactin blood test. Find out what it is and why it matters!

The FSH test evaluates the levels of Follicle-Stimulating Hormone in blood. The LH test detects the levels of luteinising hormone in the blood. The prolactin checks the levels of prolactin hormone in the blood.

What is FSH LH Prolactin Blood Test?


FSH, LH, and Prolactin Panel measures the levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and prolactin in the blood of both men and women to ensure a healthy reproductive system. These hormones are produced in the anterior pituitary gland. It helps the sex organs in both men and women during sexual development.

Why FSH LH Prolactin Blood Test Matters in Fertility?

In women, FSH and LH help in menstruation, ovulation, and puberty. Prolactin helps in the production of breast milk in women who are pregnant or breastfeeding. In men, FSH and LH stimulate the production of testosterone that is necessary for the production of sperms. The exact role of prolactin in men is unknown. However, small amounts of prolactin are produced in both men and women that help to measure sexual satisfaction in both men and women.

Fertility test

This test is performed mainly to assess the functions of reproductive organs, fertility issues, ovulation process, and the function of the pituitary gland or hypothalamus. In women, your doctor may refer you to perform this test if you exhibit irregular or heavy menstrual periods, difficulty getting pregnant or fertility issues or if you suspect menopause, and/or to detect any pituitary or hypothalamus problems.

In men, your doctor may refer you to perform this test if you exhibit low sperm count, fertility issues, or signs of low testosterone levels such as decreased sex drive, low muscle mass, and/or to detect any pituitary or hypothalamus problems.

This test result explains a diagnosis regarding infertility. The levels of each hormone help to assess the fertility issues, functions of reproductive organs, and pituitary gland or hypothalamus functions.

Fertility test for females

  1. Testosterone
  2. Estradiol
  3. Progesterone
  4. Thyroid

FSH LH and prolactin blood test preparation

Inform your doctor if you are on any medications, have any allergies or underlying medical conditions before your FSH LH and prolactin. Your doctor will give specific instructions depending on your condition on how to prepare for FSH LH and prolactin testing.

No specific preparation is required for this test. Your doctor may ask you to stop taking certain medications for up to four weeks before the test. Such as;

  • birth control pills
  • other hormone pills

If this test is being performed with any other tests such as blood sugar test, you may need to fast (not eat or drink) for up to 10 hours leading up to the test.

Introduction to the Pathology Laboratory

FSH LH prolactin blood test interpretation

If the test result falls in the normal reference range, no medical intervention is necessary.

In women, the increased levels of FSH and luteinizing hormone (LH) may show a condition called primary ovarian failure, where the ovaries cannot function properly. Many conditions, such as.

May lead to primary ovarian failure. Women with underdeveloped ovaries and women undergoing chemotherapy and radiation therapy may also exhibit increased levels of FSH and LH.

Decreased levels of FSH and LH may show a condition called secondary ovarian failure. This condition is because of the problem with pituitary or hypothalamus glands. This cannot stimulate the ovaries and ovarian function leading to ovulatory problems.

In men, the increased levels of FSH and LH may show a condition called primary testicular failure, where the tests cannot function properly. There are many causes for this condition, such as:

  • autoimmune disorders
  • some type of cancers
  • Klinefelter syndrome (a genetic abnormality)
  • viral infection such as mumps
  • trauma

In men whose testes are underdeveloped and men undergoing chemotherapy or radiation therapy also show increased levels of FSH and LH. Decreased levels of FSH and LH may show a condition called secondary testicular failure.

This condition is because of the problem with pituitary or hypothalamus glands. This cannot produce sufficient male sex hormone, testosterone affecting the development of male characteristics and fertility. Lack of sexual drive, fatigue, low muscle mass, sexual dysfunction is common in men with low testosterone levels.

Small amounts of prolactin are produced in the blood of both men and non-pregnant women. However, high levels of prolactin are normal during pregnancy and in breastfeeding women.

A moderate increase in prolactin levels may be seen in individuals with stress because of an illness, seizures, lung cancer, or chest wall trauma, etc. However, high levels of prolactin in men and non- pregnant women can be because of conditions such as;

  • liver disease
  • kidney disease
  • hypothyroidism
  • an eating disorder (anorexia nervosa)
  • hypothalamus
  • pituitary disorders
  • infertility issues
  • Polycystic ovary syndrome is also a reason for high levels of prolactin in women

Abnormally high levels of prolactin in both men and women are called prolactinoma, a condition in which a noncancerous pituitary tumour overproduces the prolactin in the body. Symptoms of prolactinoma among women are;

  • an unexplained flow of breast milk (galactorrhea)
  • visual impairment
  • a headache
  • abnormal growth of body and facial hair
  • acne
  • irregular menstrual periods
  • vaginal dryness
  • pain or discomfort during sex

Prolactinoma symptoms

  • visual impairment
  • a headache
  • abnormal growth of body and facial hair
  • erectile dysfunction
  • decreased sex drive
  • uncommonly enlarged breasts

If you have higher or lower than normal levels of FSH, LH, and prolactin in blood, consult your doctor for further instructions. Based on the test results, your doctor may advise you on medical treatments, lifestyle modifications, and further diagnostic tests.

FSH LH PROLACTIN blood test normal levels
TestGenderAge GroupNormal Value
FSHFEMALE12-48years10-15 mIU/ml
LHFEMALE12-48years7-15 mIU/ml
ProlactinFEMALE12-48years< 25 ng/ml

Cost of FSH LH PROLACTIN Profile ₹999.00

  1. What is FSH LH PRL test?

    The FSH blood test is used to help diagnose problems with sexual development, menstruation, and fertility. The LH blood test measures the amount of luteinizing hormone, which is also secreted by the pituitary gland. In women, LH levels rise at mid-cycle; within 24 to 36 hours, ovulation occurs.

  2. What is the normal range of FSH and LH?

    The reference range for FSH and LH in adult females is: The reference range for FSH in adult males is 2–12 IU/L and for LH is 2–9 IU/L.

  3. Do you need to fast for FSH and LH blood test?

    You don’t need to fast before an FSH and LH blood test.

  4. What is the prolactin normal range?

    The normal range for prolactin in your blood is: Males: 2 to 18 nanograms per milliliter (ng/mL) Nonpregnant females: 2 to 29 ng/mL. Pregnant females: 10 to 209 ng/mL.

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